FastQCFastQC Report
Thu 11 Sep 2014
SDW_SD2_3_TGACCA_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_SD2_3_TGACCA_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16318218
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAAGTGTGTTGCCGTCTTCAAGTTGCTTGCCGGCAAAGATCAAACGC261780.16042192842380215No Hit
GTAATCTTGAAGTGTGTTGCCGTCTTCAAGTTGCTTGCCGGCAAAGATCA228190.1398375729506739No Hit
CCGGACAAGAACACGTGGTACCTACGACACATATTCTGAAAGAAATGCAT213370.13075569893722463No Hit
GTCAACACGAAGTTCGTTGTAGAATGTGTGGTGCCAGATCTTTTCCATAT196830.12061978826364496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACG751.357621E-744.3609241
CCGGGAT9050.032.5616871
CTCGGTT30750.031.377241
CGGGCCC1102.723129E-630.2460841
CGGGGTT9850.029.9170861
CGGGTCG1601.897206E-929.7059781
CCTCGGG5500.029.36318614-15
CTCGGGG5600.027.99051316-17
CCGGGTT6800.026.5606371
CCCTATG5950.026.34389166-67
CCGGGCA2207.6397555E-1125.9252151
GACCCGA13150.025.64862692-93
CCGCTAT9100.025.592841
CCGGGCC1151.2773603E-424.7980331
CGGGACA8050.024.7980331
CCCGCCC1000.001648597223.7647821
CCGGTAT9350.023.3835281
CCGGGCT3100.022.9981751
CGGGGTG2103.430614E-822.6331231
CGGGGCA3400.022.3668521
Produced by FastQC (version 0.11.2)