FastQCFastQC Report
Thu 11 Sep 2014
SDW_SD2_2_TGACCA_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_SD2_2_TGACCA_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12222133
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAAAAGAAACAAGGCGTTAAGAGAATCGATCGTTGGAATATTAGTAAT239480.1959396121773507No Hit
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTA187120.15309929944306774No Hit
CACAGTTCCAATTTACGAAGGCTACTCCCTTCCACACGCCATCATGAGAC181690.14865653973819465No Hit
AAAGAAACAAGGCGTTAAGAGAATCGATCGTTGGAATATTAGTAATATGA180960.14805926265079916No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCAT160540.1313518679595452No Hit
AAGAAACAAGGCGTTAAGAGAATCGATCGTTGGAATATTAGTAATATGAA125090.10234711077027225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGGGA40600.047.945935
CAGGGAT41500.047.0207336
CGGACTA7150.043.1617934
GACCCGC17250.042.3863035
CCTCGGG18800.041.92667814-15
AGACCCG18000.040.092514
CCCTGAC25500.038.74251638-39
GCCCTAT24050.038.23365866-67
CCCTATG24600.037.95835566-67
TCGGGGA25350.037.27441416-17
GGGATTT53400.037.262168
CGTTCGA16450.037.2319684
AGCCCTA25350.036.8359364-65
CTCGGGG22100.035.4509116-17
GAGCCTA28300.034.83761674-75
CGAGCCC28200.033.365162-63
CGGACGA28450.033.2371792-93
TAGCTTA30200.032.71539752-53
CCCGCTG22900.032.3475767
AGGGATT61750.031.6051247
Produced by FastQC (version 0.11.2)