FastQCFastQC Report
Thu 11 Sep 2014
SDW_SD2_2_TGACCA_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_SD2_2_TGACCA_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12222133
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTA188700.154392036152773No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCAT169840.13896101441540523No Hit
TAAAACTTGGATTCTTCCGGACAAGAACACGTGGTACCTACGACACATAT158580.12974821988927793No Hit
ATAAAACTTGGATTCTTCCGGACAAGAACACGTGGTACCTACGACACATA136340.11155172341849005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGGGA33400.056.172245
TCCAGGG40000.046.9049724
CTCGGGG19850.043.42991616-17
CCTCGGG20050.043.35204714-15
CCCTATG24150.042.18829366-67
CAGGGAT46150.040.9621286
GCCCTAT25050.040.48293366-67
CGAGCCC26250.038.7225262-63
GGACCGG20100.038.517915
TCGGACG27200.037.63979390-91
CGGGACC1152.4901965E-937.1995351
AGCCCTA27800.036.9053964-65
CGGGGAA27750.036.79989618-19
TCGGGGA28200.036.63389216-17
GAGCCTA27100.035.1418974-75
ACTAACC29100.034.9298956-57
CCTATGC29600.034.5007568-69
CTGACCC28700.033.0993340-41
GCCTACG30050.032.3260676-77
CCGGGCT2800.032.2543561
Produced by FastQC (version 0.11.2)