FastQCFastQC Report
Thu 11 Sep 2014
SDW_SD2_1_TGACCA_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_SD2_1_TGACCA_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11437616
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAAAAGAAACAAGGCGTTAAGAGAATCGATCGTTGGAATATTAGTAAT385980.33746542985880973No Hit
AAAGAAACAAGGCGTTAAGAGAATCGATCGTTGGAATATTAGTAATATGA281890.246458702582776No Hit
CAGACACTCGTTATCGATAACGGCTCTGGTATGTGCAAGGCTGGCTTCTC206580.18061456163592132No Hit
AAGAAACAAGGCGTTAAGAGAATCGATCGTTGGAATATTAGTAATATGAA195880.1712594652591939No Hit
ATCCAACTCAAAAGAAACAAGGCGTTAAGAGAATCGATCGTTGGAATATT177570.15525088445004623No Hit
CGTCAAGACACTCACAGGCAAACACATTACACTCGAAGTCGAACCAACCG177240.15496236278609107No Hit
AAAGTATCCAACTCAAAAGAAACAAGGCGTTAAGAGAATCGATCGTTGGA149650.13084020306329572No Hit
CTTCAGTCAACTTCCCAGCACGTGAACTTTCACGCAATGTTCGCCCAACC127190.11120324375289395No Hit
AAACAAGGCGTTAAGAGAATCGATCGTTGGAATATTAGTAATATGAATGA120400.10526669193999867No Hit
CCAACTCAAAAGAAACAAGGCGTTAAGAGAATCGATCGTTGGAATATTAG114930.10048422678292399No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCCG17600.056.6481064
GACCCGC17650.056.2188845
CCCGCTG21450.045.82077
ACCCGCT24450.040.583376
CGTTCGA18550.039.67044
CGGACTA5950.035.90664
AACCTAC21600.034.5085375
CCTACTA19150.033.7200937
ACCTACT21300.030.7595636
TTCAGTC119150.027.6888482
CCTCGGG4700.026.77124614-15
CCGATCC20550.026.1063023
CTGTTAG30850.025.3982898
CCCTGAC8150.024.1828838-39
CCGCTGA42500.023.7994218
CACTCAC122250.023.6167939
CAACCTA32000.023.589894
CAGTCTC71700.023.5064984
CTGACCC15850.023.3638343
CTTCAGT150350.023.0550791
Produced by FastQC (version 0.11.2)