FastQCFastQC Report
Thu 11 Sep 2014
SDW_SD2_1_TGACCA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_SD2_1_TGACCA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11437616
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAAACTTGGATTCTTCCGGACAAGAACACGTGGTACCTACGACACATA188660.16494696097508432No Hit
CCGGACAAGAACACGTGGTACCTACGACACATATTCTGAAAGAAATGCAT169220.14795041204390844No Hit
GACAAGAACACGTGGTACCTACGACACATATTCTGAAAGAAATGCATCTG155820.13623468387118434No Hit
CTTGGATTCTTCCGGACAAGAACACGTGGTACCTACGACACATATTCTGA133930.11709608016215968No Hit
TAAAACTTGGATTCTTCCGGACAAGAACACGTGGTACCTACGACACATAT131950.11536495017842878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGGG5450.035.2939114-15
GGACCGG24300.035.180645
CTCGGGG5950.032.32789616-17
CCGGGCA2400.031.6830711
CTCGGTT21600.030.3629471
GACCGGC28350.030.322366
CCCTATG6950.029.38918966-67
CCGGGAT6150.028.5920431
CGAGCCC7800.025.57585362-63
TCGGACG8250.024.7681890-91
GCCCTAT8150.024.4791166-67
CGGGGCT4000.022.574191
CCAGGGA12650.022.5267335
ACTAACC9800.022.05226356-57
CCGCTAC13700.020.8136981
CCGGGTA2751.2532837E-920.7380121
GGCAGGG11850.020.4403976
GTTTATT95900.020.4172461
AGGGACG11950.020.2694369
CTTAGGC23100.020.16014990-91
Produced by FastQC (version 0.11.2)