FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCG3_3_GCCAAT_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCG3_3_GCCAAT_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15003086
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCT289830.19318025638192035No Hit
CTGAAGAAGACGTTCAGACACTCGTTATCGATAACGGCTCTGGTATGTGC265570.1770102497579498No Hit
CTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCTA248320.1655126152046319No Hit
CTTACATCTTCAGTCAACTTCCCAGCACGTGAACTTTCACGCAATGTTCG247240.1647927633021633No Hit
CTTACATCTTCAGTCTCTCTTCCAGCACGTGAACTCTCCCGCAAGGTTCT181380.12089512784236524No Hit
CGTGGAGCGATCAACTTGTCCAACTCAAGACAGAATCAACAGGCTGCGCT160530.10699798694748534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGAG40950.054.7221261
GTGGAGC47000.046.967262
TGGAGCG48200.045.504913
GGAGCGA49050.045.2970774
GAGCGAT51450.043.27685
AGCGATC54400.040.493096
CTGTTAG43400.035.6647348
TACTGTC36350.035.0020684
TGGAACG112900.032.126453
GGAACGA114550.031.7051374
GCGATCA70000.031.6080387
AACCTAC27850.031.5365495
CGAGCCG8650.028.5405371
CCTACTA27950.028.5388957
TTCAGTC207950.028.4019379
ACCTACT28250.028.2327186
CGTACTG14850.027.8164987
TGTTAGC58700.027.0143729
GAGGCGC26750.026.9777879
CTGACCC21300.025.6319453
Produced by FastQC (version 0.11.2)