FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCG3_3_GCCAAT_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCG3_3_GCCAAT_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15003086
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAACACGAAGTTCGTTGTAGAATGTGTGGTGCCAGATCTTTTCCATAT176310.11751582307799875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCG905.6755744E-736.9680821
CGGGACG1401.6982085E-830.555251
CCGGGTC1405.781567E-727.1602231
CGGGGGA3350.026.9575371
CCGGGGA3950.026.4726221
CCCGCGT908.919227E-426.4057731
CTCGGTT22550.026.3472251
CGGGCTA2251.0186341E-1025.3495431
CGGGGCA3200.025.2505191
CCGGGTT7300.024.7418481
GTCGGGG2501.4551915E-1124.7158031
CCCCGCT2151.6534614E-924.3178751
CGGGGCT2950.024.1679951
CTCGGGG3700.022.46568116-17
CGGGGTT8550.022.236441
CGGGTCT5200.021.9371051
CCGGGAT8050.021.8462681
CGGGGAT8050.021.8462681
CCTCGGG4000.021.37484614-15
CGGGGTG2901.05501385E-1021.3067281
Produced by FastQC (version 0.11.2)