FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCG3_2_GCCAAT_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCG3_2_GCCAAT_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13013283
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCT251760.19346386303901944No Hit
CTTACATCTTCAGTCAACTTCCCAGCACGTGAACTTTCACGCAATGTTCG211180.16228034078717876No Hit
CTGAAGAAGACGTTCAGACACTCGTTATCGATAACGGCTCTGGTATGTGC202750.15580234441992846No Hit
CTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCTA179910.13825104702633456No Hit
CCGAGAACGACGCAATCATCAAAGCAGCTATCGCCGAAGCAGCCAAGTCT136290.1047314501651889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGAG37050.051.767841
GGAGCGA42950.043.661554
TGGAGCG43700.043.129493
GTGGAGC45700.041.137472
GAGCGAT47500.039.379445
AGCGATC49050.037.9414526
TACTGTC31600.037.4091224
GCGATCA57100.032.846047
GGAACGA93850.032.7291874
CTGTTAG35650.032.36768
TGGAACG96800.031.8788953
AACCTAC24650.029.852575
TTCAGTC162350.029.2194469
ACCTACT23700.029.0460176
CCTACTA22600.028.9929667
ACTGTCG44900.027.0682725
CGAGCCG6350.026.915081
CGTAGCT12750.025.3201141
CTGACCC20850.025.2744753
CGTACTG13700.024.9536447
Produced by FastQC (version 0.11.2)