FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCG3_2_GCCAAT_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCG3_2_GCCAAT_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13013283
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCG851.625755E-533.552681
CGGGGCA2300.033.0664141
CTCGGTT18350.031.6022991
CGGGCCC1802.0918378E-1029.0479241
CGGGGCT3150.028.6706771
CCGGGTC856.3848076E-427.9605671
CCGGGTT5650.024.3974511
CGGGTTA3150.024.1437281
CCGGGCT2202.1573214E-923.7664831
CGGGACG1000.001647891823.7664831
CGGGGGA3050.023.376871
CCGGGAT7600.023.141051
CGGGGGT3500.023.0874421
CGGGCTA1954.2002284E-721.9382921
CCGGCCG1754.030244E-621.7293571
CGGGGTT7050.021.5752471
CGGGCCG1604.9677514E-520.7956711
CCGCTAT8500.020.131611
CGGGTCT4500.020.0694771
GGGGGAT5450.020.0597861
Produced by FastQC (version 0.11.2)