FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCG3_1_GCCAAT_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCG3_1_GCCAAT_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11009108
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACATCTTCAGTCAACTTCCCAGCACGTGAACTTTCACGCAATGTTCG217160.19725485479840876No Hit
CTGAAGAAGACGTTCAGACACTCGTTATCGATAACGGCTCTGGTATGTGC211740.19233165847768957No Hit
CCTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCT194960.17708973333716047No Hit
CTTACATCTTCAGTCTCTCTTCCAGCACGTGAACTCTCCCGCAAGGTTCT162290.14741430459216134No Hit
CTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCTA159730.14508895725248586No Hit
CTTTACTGTCGCCCAGATCCGCGAAGTTATGAACAAGCGTCATAACATTC121560.11041766508240267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGAG27850.051.3214651
GTGGAGC32500.042.5039372
GAGCGAT34450.040.2404175
GGAGCGA34950.039.2568634
TGGAGCG35000.039.065143
AGCGATC35850.038.5365376
CTGTTAG32300.036.9018978
TACTGTC32050.036.2913364
TTCAGTC173950.030.983839
TGGAACG75050.030.1739793
GGAACGA75800.030.1259544
GCGATCA47550.029.4578867
AACCTAC21850.029.1153625
CGAGCCG5000.027.5413821
ACTGTCG44150.027.0980915
CCTACTA21400.027.0692447
ACCTACT22100.026.8526176
TACATCT162750.026.7493523
GAGGCGC17600.026.7107589
CGTTCGA14800.026.624494
Produced by FastQC (version 0.11.2)