FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCG3_1_GCCAAT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCG3_1_GCCAAT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11009108
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAACACGAAGTTCGTTGTAGAATGTGTGGTGCCAGATCTTTTCCATAT131950.1198553052617887No Hit
GCCAGATCTTTTCCATATCATCCCAGTTGTTGACGATACCGTGTTCAATT110770.10061668938119238No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCG801.074258E-535.6452371
CTCGGTT14800.032.4339521
CGGGGTA2850.026.6818141
CGGGGCT3250.024.8602681
CCCGTGT2252.8012437E-923.2354131
CGGGGAT6050.022.781531
CGGGGTT6650.022.1554361
CATTACC45650.021.744329
GACTCCG24250.021.54580352-53
CTCGGGA3101.0913936E-1121.4637991
CGGGTGT10400.021.021551
AAAGCTA35150.020.6734224
CGGGTCT4150.020.6141131
CCGGGAT7650.020.5018351
CCGCTAT7750.020.2372971
ACTCCGT23950.020.03076752-53
CTCGGGT4050.019.9495981
TCATTAC52600.019.6838978
GTTTAGT52050.019.1751521
CGGGGCA2001.2800869E-519.0107941
Produced by FastQC (version 0.11.2)