FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCF20_3_GTCCGC_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCF20_3_GTCCGC_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18047500
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGTTCCAATTTACGAAGGCTACTCCCTTCCACACGCCATCATGAGAC314350.1741792492034908No Hit
CAGACACTCGTTATCGATAACGGCTCTGGTATGTGCAAGGCTGGCTTCTC295660.1638232442166505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCGC24300.055.0953565
AGACCCG26250.051.1832774
CGGACTA8350.042.6428034
CGTTCGA30200.041.8163034
CCCGCTG32650.041.736677
ACCCGCT35450.038.167596
AACCTAC26400.038.1244935
ACCTACT27150.033.9232946
CCTACTA27450.032.1728637
CTGACCC30200.028.7689173
CTGTTAG53750.028.7097918
TAGTGCG10050.028.3442233
CTTTGAC90000.026.904811
TTCAGTC149400.025.4538842
CCTCGGG8950.024.93473614-15
CACTCAC131900.024.7294359
CAACCTA43400.024.1753884
GCCCGAC5550.023.9517485
TTTGACT99150.023.3189182
CCGATCC28850.022.0515123
Produced by FastQC (version 0.11.2)