FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCF20_3_GTCCGC_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCF20_3_GTCCGC_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18047500
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCCC1900.035.0230871
CCGGGAT11550.031.6875551
CCCTATG7850.031.46408866-67
CTCGGTT30750.028.2869381
CCTCGGG8300.026.61129214-15
CTCGGGG8600.025.40640816-17
CGGGGGT6100.024.9344711
CCGGGTT8400.024.8973661
GGACCGG13200.024.4688115
GCCCTAT10300.023.5187666-67
CGGGGTT10500.022.6339661
CCCGGCT3101.0913936E-1121.4657631
CCCGGAT5650.021.0315631
CCGTCCA37300.020.7566784
CACAGTT118850.020.1963181
CGGGGAT10650.020.0836621
CCGCTAC39150.019.9109551
TCGGACG12450.019.84006790-91
GGCAGGG18200.019.0515086
CCGGGGT3557.8216544E-1118.7447531
Produced by FastQC (version 0.11.2)