FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCF20_2_GTCCGC_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCF20_2_GTCCGC_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21462530
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACACTCGTTATCGATAACGGCTCTGGTATGTGCAAGGCTGGCTTCTC434420.20240856972593632No Hit
CACAGTTCCAATTTACGAAGGCTACTCCCTTCCACACGCCATCATGAGAC377360.1758227012379249No Hit
CTTCAGTCAACTTCCCAGCACGTGAACTTTCACGCAATGTTCGCCCAACC225980.10529047600632357No Hit
CTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCTATCATCACACAACA216170.10071971943661814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCGC29450.050.137265
CGTTCGA42300.048.7117654
AGACCCG31300.047.1737634
CGGACTA11700.042.6076854
CCCGCTG37800.038.9419027
ACCCGCT44600.033.745046
AACCTAC37000.031.1810045
CTGTTAG64600.030.8005988
GCCCGAC6800.029.324185
ACCTACT36250.028.6828046
CCTACTA35650.027.970947
CCTCGGG10700.026.84702114-15
CACTCAC164600.026.1376749
CCGATCC36500.025.494573
TTCAGTC194200.025.3270682
CCCTGAC17200.024.8535838-39
CAGGGAT35950.023.9037026
CTGACCC35800.023.6059513
CTCGGGG12850.022.35508216-17
CGAGCCG8850.021.9944931
Produced by FastQC (version 0.11.2)