FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCF20_2_GTCCGC_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCF20_2_GTCCGC_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21462530
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTT42150.035.9719771
CCCTATG12650.034.92048366-67
CCTCGGG11850.033.2685514-15
CTCGGGG11700.032.88310216-17
GCCCTAT13950.031.66624566-67
CCGGGAT12250.027.936221
CCGGGTT8750.027.1602121
CGAGCCC17200.025.82060662-63
TCGGACG18000.024.94082890-91
AGCCCTA18700.023.62254164-65
AGGGACG24200.023.3564199
GGCAGGG24600.023.1696666
CGGGGTT12600.022.633511
CCAGGGA29750.021.8729445
ACTAACC20150.021.80451456-57
CACAGTT129500.021.6180631
CCCGGAT4250.021.2488731
TCGGGGA22000.021.05016716-17
CGGGGAA21700.021.0128618-19
GGACCGG14700.021.0024365
Produced by FastQC (version 0.11.2)