FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCF20_1_GTCCGC_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCF20_1_GTCCGC_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14775267
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGTTCCAATTTACGAAGGCTACTCCCTTCCACACGCCATCATGAGAC218440.14784165998489232No Hit
CTTTGACTCATGAGAGAGAAGCTGAGGCGAAGGCGTCTACCTAGAGGGTT179230.12130406848146974No Hit
CAGACACTCGTTATCGATAACGGCTCTGGTATGTGCAAGGCTGGCTTCTC155910.10552093576380042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCGC19950.052.116995
AGACCCG22350.046.520384
CCCGCTG25100.040.8605087
CGTTCGA30500.039.693284
ACCCGCT26800.039.5046466
CGGACTA7850.036.892394
AACCTAC21500.032.6814545
CCTCGGG9000.030.33499314-15
CCGGGAT6300.028.6410431
CCTACTA21550.027.761767
ACCTACT22900.027.159016
CACTCAC114450.026.1387799
CTTTGAC90450.025.6186961
TCGGGGA12250.025.1908116-17
CCCTGAC14600.025.04585638-39
GCCCGAC3850.024.6630845
CGGGCAT8150.024.4701981
TTTGACT100450.024.1485022
GCCCTAT12900.024.1255866-67
CCCTATG12950.023.8489866-67
Produced by FastQC (version 0.11.2)