FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCF20_1_GTCCGC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCF20_1_GTCCGC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14775267
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTATG9500.036.5016266-67
CCGGGAT8900.036.312611
CTCGGTT28550.033.7931371
CCTCGGG9850.033.2696814-15
GCCCTAT10900.032.03124266-67
CTCGGGG10100.031.9758316-17
CCCGGCT3550.030.7920131
AGCCCTA13200.026.98910764-65
CGGGCCC2800.025.460791
TCGGACG13950.025.03718890-91
AGGGACG19600.024.2314669
CGAGCCC14550.024.15775962-63
GGCAGGG19300.024.1158756
CCGGGCT3200.023.7634031
CGGGGCT4550.022.9799921
GGGGACT17950.022.7561683
CCGGTAT14350.022.5214121
CCGGGTT6550.022.4936031
CCAGGGA25950.022.1453385
CGGGGTT8200.022.0246161
Produced by FastQC (version 0.11.2)