FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCE32_8_3_AGTCAA_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCE32_8_3_AGTCAA_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15078497
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACATCTTCAGTCAACTTCCCAGCACGTGAACTTTCACGCAATGTTCG316260.20974239010691847No Hit
CCTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCT259210.1719070541314562No Hit
CTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCTA253320.1680008292603699No Hit
CTGAAGAAGACGTTCAGACACTCGTTATCGATAACGGCTCTGGTATGTGC232650.15429256642754247No Hit
CAAGCATTCAACAAGAGCAAGAAAGTTCAGGCATTCGTCGACGAGTTCGT225570.14959713822936066No Hit
CTTACATCTTCAGTCTCTCTTCCAGCACGTGAACTCTCCCGCAAGGTTCT224440.1488477266666565No Hit
CGTTAAGACCCTTACAGGCAAGCACATCACACTCGAAGTCGAGCCAACAG190030.126027149788205No Hit
CTTCAGTCAACTTCCCAGCACGTGAACTTTCACGCAATGTTCGCCCAACC171960.11404319674567034No Hit
CCACATTCTCATCACTGGTGCTGCTGGTCAAATCGGCTACGCTCTTACAT162660.10787547326500779No Hit
CAAGCTTACACTTCACGACCTTACAGAGAAAGATCTCGCCGGCAAGCGCA159580.10583282935958405No Hit
CAGGCATTTAACAAGAGCAAGAAAGTTCAGGCATTCGTCGACGAGTTCGT156860.10402893604050856No Hit
CACATTCTCATCACTGGTGCTGCTGGTCAAATCGGCTACGCTCTTACATT154120.10221177879996925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGAG40100.048.309661
GTGGAGC46650.042.542042
GGAGCGA46400.042.3628044
TGGAGCG47250.041.7014853
CCTACTA28750.041.4563947
AACCTAC33950.039.5782175
GAGCGAT50800.038.5072985
ACCTACT31950.038.0422066
AGCGATC54200.036.6162226
GTCTGAG24600.034.163031
CTGTTAG39800.033.1680768
TACTGTC34500.032.2032054
GCGATCA63350.031.5566717
CGAGCCG7450.031.2289961
GACCCTT68900.031.1512097
CTGACCC19450.030.2696443
TTCAGTC242050.029.1110959
GCGGTCG14550.029.041612
GGAACGA116550.028.88264
TGGAACG118500.028.3273743
Produced by FastQC (version 0.11.2)