FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCE32_8_3_AGTCAA_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCE32_8_3_AGTCAA_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15078497
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAATTCGTCGAAGAATTTAGAAGCCACCACGAAGACGAAGAACGAGGT166490.11041551422532364No Hit
GTCAACACGAAGTTCGTTGTAGAATGTGTGGTGCCAGATCTTTTCCATAT162660.10787547326500779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGCA3100.038.3309481
CGGGGTA3300.034.5675471
CTCGGTT21650.034.028671
CCGGGTC702.0468082E-433.9502681
CGGGGGT2950.030.6127851
CGGGTCT5150.030.4563561
CCCGCTA5000.029.4688321
CGGGTCG1303.0240335E-729.249461
CCCGGCT1652.6357156E-928.8062881
CGGGGTG3000.028.518221
CCGGGCA2201.8189894E-1228.086131
CTCGGGC2450.027.1602151
CACAGGT11550.027.1602131
CCGCTAT11050.026.6686271
CGGGGTT7850.026.0357461
CGGGCCG1309.981963E-625.5932771
AAAGCTA55650.024.4959374
ATTCGTC76450.023.4849035
CCCGGAT3700.023.1228851
CTCGGGA6000.022.9730131
Produced by FastQC (version 0.11.2)