FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCE32_8_2_AGTCAA_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCE32_8_2_AGTCAA_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13518259
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTAAGACCCTTACAGGCAAGCACATCACACTCGAAGTCGAGCCAACAG275050.20346554981673307No Hit
CTTACATCTTCAGTCAACTTCCCAGCACGTGAACTTTCACGCAATGTTCG236180.1747118471394874No Hit
CTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCTA214060.15834879328765636No Hit
CCTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCT202210.14958287158131828No Hit
CTTACATCTTCAGTCTCTCTTCCAGCACGTGAACTCTCCCGCAAGGTTCT201510.1490650534214502No Hit
CTGAAGAAGACGTTCAGACACTCGTTATCGATAACGGCTCTGGTATGTGC168740.1248237661373406No Hit
CAAGCATTCAACAAGAGCAAGAAAGTTCAGGCATTCGTCGACGAGTTCGT163990.12131000005252157No Hit
CTTCAGTCAACTTCCCAGCACGTGAACTTTCACGCAATGTTCGCCCAACC152820.11304710170148391No Hit
CTTCGTTAAGACCCTTACAGGCAAGCACATCACACTCGAAGTCGAGCCAA146350.10826098242384616No Hit
CCACATTCTCATCACTGGTGCTGCTGGTCAAATCGGCTACGCTCTTACAT142400.10533900852173347No Hit
CACATTCTCATCACTGGTGCTGCTGGTCAAATCGGCTACGCTCTTACATT141280.10451049946594454No Hit
CAAGCTTACACTTCACGACCTTACAGAGAAAGATCTCGCCGGCAAGCGCA140220.1037263748238586No Hit
CAACAACAACACTTCGAGCACGATTGCACAGCTGCTGCCCACAAGATTAA137710.10186962685061739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGAG35450.048.749941
CCTACTA25900.045.098087
ACCTACT28100.042.5770036
GTGGAGC41500.041.8702772
GGAGCGA41200.041.484534
AACCTAC31200.040.7813035
TGGAGCG43250.039.847533
GAGCGAT46200.037.6114435
AGCGATC47350.036.89856
TACTGTC28850.034.3938944
GACCCTT87650.033.207127
CTGTTAG33450.031.7989798
GCGATCA55450.031.3402357
TAAGACC93550.030.551574
AGACCCT102800.028.4490976
CAACCTA45050.028.1382684
GACCCGC21600.027.9145645
GGAACGA97250.027.6317064
ACGACGC60100.027.5723427
TTCAGTC209550.027.4189599
Produced by FastQC (version 0.11.2)