FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCE32_8_2_AGTCAA_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCE32_8_2_AGTCAA_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13518259
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAATTCGTCGAAGAATTTAGAAGCCACCACGAAGACGAAGAACGAGGT240650.17801848596035924No Hit
CTTGATTTCATTACCTTCTTTCTTAACGAAGAAAAGAGCTGGAATTGAAG166580.12322592724403343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTT18500.040.3375631
CCCGGCT853.6256642E-739.1436121
CGGGGCA3050.037.4018551
CGGGTCG801.0735732E-535.648641
CGGGACG958.664065E-735.0232281
CATTACC52300.031.4231159
CCCTATG3400.029.33794466-67
ATTCGTC87300.029.0537245
CGTCGAA89600.028.3609398
CACAGGT16200.028.1668281
TCATTAC62900.026.8827788
CGGGTAT6750.026.7584861
CCGGGTT6750.026.7584861
AAAGCTA49050.026.3400124
GACTCCG32850.025.59278352-53
TTCGTCG103400.025.218936
CGGGGAT7000.025.1238061
CGGGGGT3050.024.934571
AAATTCG104000.024.8467333
CCGGGCA2751.8189894E-1224.1978661
Produced by FastQC (version 0.11.2)