FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCE32_8_1_AGTCAA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCE32_8_1_AGTCAA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8458150
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAACACGAAGTTCGTTGTAGAATGTGTGGTGCCAGATCTTTTCCATAT101790.1203454656160035No Hit
GTAAATTCGTCGAAGAATTTAGAAGCCACCACGAAGACGAAGAACGAGGT98050.11592369489782045No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCCC801.0747963E-535.6424871
CTCGGTT9750.034.6067241
CGGGGGA2250.033.794361
CGGGTCG1151.03325874E-733.05971
CCCGCTA3050.032.7209741
CCCGCGT600.00394287731.682211
CGGGGGT1601.899025E-929.7020741
CGGGGTG1303.027708E-729.245121
CCCTATG2500.027.55138266-67
CGGGGAT4000.027.3259071
CCGGGCC908.926656E-426.4018441
CCGCTAT6150.024.7275811
CCCGGGT1450.024.57397780-81
CGGGTCT2950.024.1643981
CGGGGCA1201.7063826E-423.7616581
CATTACC33800.023.3255049
GTATACC18650.023.1762563
GCCGCTA7350.022.6301521
GCCCTAT3150.022.62018266-67
GACTCCG17800.022.54795652-53
Produced by FastQC (version 0.11.2)