FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCD15_3_CCGTCC_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCD15_3_CCGTCC_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18753627
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGTTCCAATTTACGAAGGCTACTCCCTTCCACACGCCATCATGAGAC399150.21283882845702326No Hit
CAGACACTCGTTATCGATAACGGCTCTGGTATGTGCAAGGCTGGCTTCTC202930.10820840150014716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCGC25850.055.1017345
AGACCCG27550.052.2182244
CCCGCTG34400.041.9610527
CCTGACG25850.041.692536
CGGACTA7500.040.515374
ACCCGCT35800.040.4491846
AACCTAC23150.035.276225
ACCTACT21950.034.8244556
CGTTCGA27850.032.22094
ACCTGAC36250.030.6487245
CCTACTA25100.029.7000477
GACGTAC37000.029.514579
CCTCGGG10950.029.48708314-15
GACCTGA39900.028.0827874
GCCCTAT13200.027.18517166-67
TCGGGGA13450.026.83161716-17
TAGTGCG8550.026.0999573
CCCTGAC15050.026.03618238-39
CGAGCCC13950.025.89392562-63
CCCTATG13800.025.65879666-67
Produced by FastQC (version 0.11.2)