FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCD15_3_CCGTCC_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCD15_3_CCGTCC_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18753627
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGTTCCAATTTACGAAGGCTACTCCCTTCCACACGCCATCATGAGAC243460.12982022090980055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTATG11400.035.62423766-67
CCTCGGG9100.035.49445314-15
CTCGGGG9500.033.74932516-17
GCCCTAT12200.033.4828966-67
AGCCCTA12450.033.00099664-65
CGAGCCC13150.031.24428762-63
CTCGGTT28500.027.0150171
CGGGCTA2007.1486284E-1026.1395281
TCGGACG15550.026.11913590-91
CCAGGGA28400.024.5853165
TCGGGGA16400.024.47359316-17
CCGGGAT9400.024.2688081
CGGGGAA16450.024.25447818-19
CCGGGTT6650.023.5845381
ACTAACC18350.022.13129656-57
CCTATGC18850.021.92257968-69
AGGGACG18150.021.4593969
CCGGGTA3800.021.2618181
CACAGTT153800.020.5185571
GAGCCTA18600.019.7911974-75
Produced by FastQC (version 0.11.2)