FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCD15_2_CCGTCC_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCD15_2_CCGTCC_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13895431
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGTTCCAATTTACGAAGGCTACTCCCTTCCACACGCCATCATGAGAC194720.14013239315858572No Hit
CAGACACTCGTTATCGATAACGGCTCTGGTATGTGCAAGGCTGGCTTCTC192010.13818211180351297No Hit
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTA155010.11155465418812846No Hit
CTTCAGTCAACTTCCCAGCACGTGAACTTTCACGCAATGTTCGCCCAACC153870.11073424062916797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCCG25250.051.7108044
GACCCGC25300.051.608615
CAGGGAT32250.047.4064836
CCTGACG17050.044.55616
CCAGGGA35900.042.3219345
CCCGCTG31200.042.3122257
CCTCGGG17750.040.92893214-15
CGGACTA6550.039.8686334
ACCCGCT34550.038.7536476
CGAGCCC22100.037.95112262-63
CTCGGGG19750.036.9045716-17
TCGGGGA23000.036.7477816-17
GCCCTAT23200.036.45803566-67
CCCTGAC21400.035.7342638-39
CCCTATG23900.035.68845766-67
CCTACTA19050.035.646497
AACCTAC21100.035.5536845
ACCTACT19850.035.4007726
CGTTCGA22900.035.0397644
AGCCCTA24500.034.62162864-65
Produced by FastQC (version 0.11.2)