FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCD15_2_CCGTCC_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCD15_2_CCGTCC_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13895431
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTA156600.11269891520457335No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCAT142420.1024941219887314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGGGA31100.054.0645835
TCCAGGG35050.047.8374064
CCTCGGG16350.041.83326314-15
CCCTATG18950.041.35852466-67
CTCGGGG16500.041.3086616-17
CGAGCCC19750.039.80270862-63
GCCCTAT20000.039.0684566-67
AGCCCTA20700.037.97621564-65
TCGGACG20750.036.9757290-91
CAGGGAT47700.036.0462576
ACTAACC22500.035.3599556-57
CCTATGC22200.035.19680468-69
TCGGGGA23450.033.9269716-17
CGGGGAA23900.032.9898418-19
GCCTACG26750.032.5852776-77
CGGGCTA1900.032.5201151
GAGCCTA26850.031.93115474-75
CGGGACG1252.1451706E-730.4188161
CTCGGTT21450.029.9136051
CCGGGAT10400.029.7058731
Produced by FastQC (version 0.11.2)