FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCD15_1_CCGTCC_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCD15_1_CCGTCC_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27136551
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACACTCGTTATCGATAACGGCTCTGGTATGTGCAAGGCTGGCTTCTC405790.14953632095692632No Hit
GAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCTATCA289400.1066458298256105No Hit
CTTCAGTCAACTTCCCAGCACGTGAACTTTCACGCAATGTTCGCCCAACC280610.10340665621065845No Hit
CACAGTTCCAATTTACGAAGGCTACTCCCTTCCACACGCCATCATGAGAC280470.10335506527708697No Hit
CGATTGTATACTACAGACGCTTCGCTCCAATGCGCTCTGGTGTCTTTGAT280080.10321134767642358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCGC50000.048.4237865
AGACCCG52850.046.081884
CCCGCTG57950.042.19587
CGTTCGA45450.042.094854
ACCTACT45650.042.01456
CCTACTA45450.041.5781027
AACCTAC49150.040.4714785
CGGACTA16150.037.3326644
ACCCGCT67350.036.3018046
CCTGACG31150.034.9008526
CTGTTAG69800.028.506238
CGTACTG30150.028.0316187
GACGTAC41450.027.8391349
CAACCTA74300.027.6666684
TTTGACT160350.024.9549272
CTGACCC34000.024.8541013
TAGTGCG21850.024.769113
CGTGGAG59700.024.6566961
CGCGGTC38000.024.2418441
CTGACGT48200.023.4447387
Produced by FastQC (version 0.11.2)