FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCD15_1_CCGTCC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCD15_1_CCGTCC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27136551
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTT45400.032.451871
CGGGGCA4850.030.3776261
CCGGGAT16000.030.0009671
CCCTATG7100.028.43444366-67
CCGGGCT4350.028.4065131
CCTCGGG6350.026.92566514-15
CCGGCCG3750.025.3473531
CGGGGAT10850.024.0916671
CTCGGGG6950.023.91778416-17
CCGGGTC2002.046363E-823.7631441
AGGGACG37100.023.2988439
CGCGGGG2452.9649527E-1023.278181
GGCAGGG37950.022.902196
CCGGGTT10150.022.4754831
CGGGTCA5100.022.3653131
CGAGCCC9650.022.15034762-63
GCCCTAT9350.021.59192866-67
CGGGGCT11100.021.4082371
CGGGGGT6900.021.3523881
CCGGGGT5400.021.1227951
Produced by FastQC (version 0.11.2)