FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCC37_3_ATGTCA_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCC37_3_ATGTCA_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9291284
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACACTCGTTATCGATAACGGCTCTGGTATGTGCAAGGCTGGCTTCTC130440.14038963828895984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCGC15000.051.9077075
AGACCCG15350.050.7241484
CGTTCGA18250.050.4682354
CCCGCTG19450.041.0117877
ACCCGCT19850.039.702476
CCAGGGA18750.034.942755
CAGGGAT18900.034.9158746
CTGTTAG26300.032.4971438
CCTCGGG7600.031.23718814-15
TCGGGGA8900.031.21112816-17
CGAGCCC8700.031.1394662-63
AACCTAC13150.031.0493135
CCCTGAC10650.030.10334638-39
CTCGGGG8100.029.6039716-17
CGGACTA3400.029.3237614
GCCCTAT9350.028.97538866-67
ACCTACT12500.028.8651316
CCTACTA12800.027.0789577
CCCTATG11000.025.06116766-67
TAGTGCG5450.024.3917623
Produced by FastQC (version 0.11.2)