FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCC37_3_ATGTCA_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCC37_3_ATGTCA_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9291284
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCCC8700.039.30934562-63
CCTCGGG8050.038.94373714-15
CCGGGAT5400.037.8479581
CTCGGGG8100.037.82331516-17
CCAGGGA15250.036.130035
CCCTATG9500.035.99927566-67
GCCCTAT10650.031.4430366-67
ACTAACC11600.029.4818556-57
TCGGACG12050.029.36805290-91
TCCAGGG18850.029.2300244
CTAACCG12800.026.71792658-59
CGGGGAA13000.026.4897118-19
CCTATGC12950.026.22534268-69
TCGGGGA13400.026.0535816-17
CCGGGTT3500.025.8019941
AGCCCTA13550.025.06393664-65
GCCTACG13350.022.41594376-77
GGGGAAT16300.022.29237718-19
CTCGGTT14550.021.8866631
CAGGGAT27100.021.032566
Produced by FastQC (version 0.11.2)