FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCC37_2_ATGTCA_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCC37_2_ATGTCA_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16477884
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACACTCGTTATCGATAACGGCTCTGGTATGTGCAAGGCTGGCTTCTC208430.12649075573052948No Hit
CACAGTTCCAATTTACGAAGGCTACTCCCTTCCACACGCCATCATGAGAC192270.1166836712772101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCGC24500.049.801315
CGTTCGA37550.047.6654664
AGACCCG26900.045.3579334
CAGGGAT33950.038.4562236
CCCGCTG32350.038.1615147
CCTCGGG13400.037.73659514-15
ACCCGCT33400.036.6730426
CCAGGGA35400.035.942255
CGAGCCC17550.033.7122462-63
TCGGGGA18350.032.3439616-17
CCCTGAC20200.032.09639438-39
GCCCTAT18000.031.81217666-67
CCCTATG19400.030.49625266-67
CGAGCCG4750.029.9860021
TAGTGCG9900.029.7323283
CTCGGGG16800.029.67558516-17
CTGTTAG44100.029.6113958
AGCCCTA19950.029.418264-65
GCCCGAC4600.028.8984095
CGGACTA6500.027.755094
Produced by FastQC (version 0.11.2)