FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCC37_2_ATGTCA_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCC37_2_ATGTCA_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16477884
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGGGA27700.042.867645
CCTCGGG14250.039.49827214-15
CTCGGGG14350.038.72617316-17
CGAGCCC17000.038.4183162-63
CCGGGAT8500.035.7909431
CCCTATG18500.035.43195766-67
GCCCTAT19050.034.28431766-67
TCCAGGG35900.032.945444
TCGGACG21450.030.89560190-91
CGGGGAA20950.030.833618-19
TCGGGGA21400.030.29629516-17
CCTATGC22150.029.27161668-69
ACTAACC22700.028.98018556-57
CCGGGCT3050.028.053351
AGCCCTA24200.027.0863764-65
GGCAGGG15400.026.5244926
CAGGGAT45800.026.341326
GCCTACG23850.025.89185576-77
CTCGGTT29650.025.81151
CGGGCTA2451.0913936E-1125.222571
Produced by FastQC (version 0.11.2)