FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCC37_1_ATGTCA_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCC37_1_ATGTCA_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5135174
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA9550.048.2141954
CGGACTA1750.037.9748954
GACCCGC5950.034.305055
CTGACCC7600.033.103113
AACCTAC7450.029.9466445
CCTACTA5650.029.4084937
AGACCCG7100.028.74864
CTGTTAG11600.025.7879128
TAGTGCG3350.025.5055243
CGAGCCG1501.0649055E-625.3235071
ACCTACT7500.025.3165956
ACCCGCT8500.024.571996
CCCGCTG8150.024.4649777
ACGGACC4300.024.2827192
CCGGGAT2801.8189894E-1223.740791
AGCCCTA16650.023.3779376
GTCGGGT1050.002202209622.6102751
GATAGTC5550.022.237555
CGTACTG5850.021.9109427
CGGGTCC10300.021.6604373
Produced by FastQC (version 0.11.2)