FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCC37_1_ATGTCA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCC37_1_ATGTCA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5135174
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCTA651.3238944E-436.561561
CCCGGCT702.0467074E-433.9500241
CGGGGGT1303.0235606E-729.249251
CCGGGTT2305.456968E-1226.8648011
CCGGCCG1257.3612046E-626.616821
CTCGGGT1601.8514438E-623.7650171
CCGCTAC11150.023.4453071
CGGGGCT2853.6379788E-1223.3480851
CCGGTAT4000.022.5767671
GTCGGGT1302.930879E-421.9369371
CGGTTCA5250.021.712783
CCGGGAT3401.8189894E-1220.9691311
CCGTCCA10300.020.7497774
CGGGTAT2108.5674947E-720.3700141
CGGGGGA1656.297488E-520.1642571
TGCGGGG1951.0350161E-519.4845039
CTCGGTT8300.019.4701331
GGACCGG3203.9835868E-1019.2942245
CGGGTCT2251.6598606E-619.0120141
CGGGTTT3557.8216544E-1118.7442381
Produced by FastQC (version 0.11.2)