FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCB20_3_CTTGTA_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCB20_3_CTTGTA_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9355190
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCTA249960.26718858729753214No Hit
CCTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCT226040.24161989227370048No Hit
CTGAAGAAGACGTTCAGACACTCGTTATCGATAACGGCTCTGGTATGTGC190210.20332029600681548No Hit
CTTACATCTTCAGTCTCTCTTCCAGCACGTGAACTCTCCCGCAAGGTTCT174590.18662368161416282No Hit
CCACATTCTCATCACTGGTGCTGCTGGTCAAATCGGCTACGCTCTTACAT165180.17656509381423574No Hit
CACATTCTCATCACTGGTGCTGCTGGTCAAATCGGCTACGCTCTTACATT163840.17513273380872008No Hit
CTTACATCTTCAGTCAACTTCCCAGCACGTGAACTTTCACGCAATGTTCG151210.16163220629404643No Hit
CGACAACCTACTACGACGAGAAATCCGGTTTCAGGATGCAGATCCACTTC125470.1341180670836188No Hit
CAAGCATTCAACAAGAGCAAGAAAGTTCAGGCATTCGTCGACGAGTTCGT117340.12542770376657233No Hit
CAAGCTTACACTTCACGACCTTACAGAGAAAGATCTCGCCGGCAAGCGCA106950.11432156909693977No Hit
GTTAAAGTTGCTATCAACGGTTTCGGCAGAATTGGCCGTCTTGTTTTCCG106250.11357332133286444No Hit
TCCACATTCTCATCACTGGTGCTGCTGGTCAAATCGGCTACGCTCTTACA101930.10895556370314231No Hit
GGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCTATC101730.10874177862769223No Hit
GAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCTATCA101400.10838903325319955No Hit
CTTCAGTCAACTTCCCAGCACGTGAACTTTCACGCAATGTTCGCCCAACC100020.10691391623259389No Hit
CTCACATCTGTTAGCTATCCAGTCCGCAACATCTGCAGAAGCAAGCTCCC99200.1060373974232485No Hit
CTTCAGTCTCTCTTCCAGCACGTGAACTCTCCCGCAAGGTTCTCCCAACC99110.10594119413929594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGAG27200.051.66931
CCTACTA26100.049.6681947
AACCTAC27850.049.098715
GTGGAGC29000.046.6602822
ACCTACT28250.046.3859526
GGAGCGA29850.044.536314
TGGAGCG32700.041.235923
AGCGATC34500.039.2212686
CCGATCC18900.038.938123
GAGCGAT34550.038.4780275
TACTGTC22300.037.2592124
CAACCTA39550.035.8942764
CCTGACG13700.035.695426
GTCTGAG13450.033.5359841
CTGTTAG31950.033.143418
GGTAGTT18450.032.167951
GACGTAC15650.032.1609739
CGCGGTC8800.031.8331131
GCGATCA42750.031.3234217
TACTACG44450.030.2309889
Produced by FastQC (version 0.11.2)