FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCB20_3_CTTGTA_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCB20_3_CTTGTA_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9355190
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGATTTCATTACCTTCTTTCTTAACGAAGAAAAGAGCTGGAATTGAAG244730.2615981075745121No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTACC58650.044.299469
TCATTAC64000.040.8187338
CGGGTCT3300.037.4508131
CGGGGCA1650.037.4508131
CGGGTCG651.3233727E-436.5644041
CGGGACG702.0459015E-433.9526631
CGGGGTG1553.6379788E-1133.7336121
CCGGGCC1001.293316E-633.273611
CGGGTTA1456.6029315E-1032.781881
CTCGGTT16400.032.4620551
CCCGGAT1051.8930787E-631.6891521
CCGGTCT3350.031.2161791
GTCGGGG1303.0219599E-729.2515221
AAAGCTA30750.028.5762834
CCCTATG1750.028.49950266-67
CCGGGTT5250.028.0675331
CGGGCCT9000.027.4639321
CCGCTAT6900.026.8668881
CGGGGAT4500.026.4076271
CGGGCTA908.9153234E-426.4076251
Produced by FastQC (version 0.11.2)