FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCB20_2_CTTGTA_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCB20_2_CTTGTA_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18417263
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCT453850.24642640983082012No Hit
CTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCTA392540.21313699000769007No Hit
CTTACATCTTCAGTCAACTTCCCAGCACGTGAACTTTCACGCAATGTTCG332610.1805968671892235No Hit
CTGAAGAAGACGTTCAGACACTCGTTATCGATAACGGCTCTGGTATGTGC307090.16674030229138825No Hit
CTTACATCTTCAGTCTCTCTTCCAGCACGTGAACTCTCCCGCAAGGTTCT296140.16079479345003653No Hit
CGACAACCTACTACGACGAGAAATCCGGTTTCAGGATGCAGATCCACTTC254670.13827787549105425No Hit
CCACATTCTCATCACTGGTGCTGCTGGTCAAATCGGCTACGCTCTTACAT229640.12468736532675892No Hit
CTCACATCTGTTAGCTATCCAGTCCGCAACATCTGCAGAAGCAAGCTCCC228370.1239977948949309No Hit
CACATTCTCATCACTGGTGCTGCTGGTCAAATCGGCTACGCTCTTACATT213350.11584240285866582No Hit
CAAGCATTCAACAAGAGCAAGAAAGTTCAGGCATTCGTCGACGAGTTCGT202510.10995662059014959No Hit
CTTTACTGTCGCCCAGATCCGCGAAGTTATGAACAAGCGTCATAACATTC188550.10237677552848107No Hit
CGTGGAGCGATCAACTTGTCCAACTCAAGACAGAATCAACAGGCTGCGCT186690.10136685347871721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACTA50300.050.1282047
AACCTAC53650.049.64735
ACCTACT52350.048.4315386
CGTGGAG62250.047.4399341
GTGGAGC72900.040.834272
GGAGCGA72050.040.724174
TACTGTC50500.040.145264
AGCGATC74800.039.481396
TGGAGCG77150.038.0321043
GAGCGAT79450.036.931515
CTGTTAG65000.036.748618
CAACCTA73800.036.284454
CCTGACG43500.035.4729846
GCGATCA87150.033.67267
CCGATCC36100.032.0907863
TGGAACG172850.031.698173
GGAACGA173900.031.2337554
ACTGTCG68950.030.6427865
TGACCTG52350.030.2919733
TTCAGTC264950.030.2892849
Produced by FastQC (version 0.11.2)