FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCB20_2_CTTGTA_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCB20_2_CTTGTA_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18417263
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGATTTCATTACCTTCTTTCTTAACGAAGAAAAGAGCTGGAATTGAAG481220.26128746708997963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTACC114000.042.3312349
TCATTAC127900.038.2505388
CGGGACG1650.037.451771
CGGGTTA3500.032.5953871
CCCTATG6150.029.73518666-67
CGGGTCG1951.6370905E-1129.2522721
CTCGGTT29400.027.6478731
CCCGGCT1903.929017E-1027.520231
CGGGTGT11950.027.049171
CCCGGAT3550.026.7802451
CCTCGGG5950.026.74273314-15
CGGGGAT10700.026.6551061
CGGGCCC1806.661139E-926.4082981
CTCGGGG6150.025.48671316-17
GCCCTAT7200.025.39880466-67
AAAGCTA59000.025.1983134
CCGGGAT8700.024.5870381
GTCGGGG2551.8189894E-1124.2334981
CGGGGTA3550.024.102221
AGCCCTA7550.023.90668364-65
Produced by FastQC (version 0.11.2)