FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCB20_1_CTTGTA_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCB20_1_CTTGTA_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11548640
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAGAAGACGTTCAGACACTCGTTATCGATAACGGCTCTGGTATGTGC277880.240617076989152No Hit
CCTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCT276150.239119065102038No Hit
CTGGAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCTA273180.2365473337120215No Hit
CTTACATCTTCAGTCAACTTCCCAGCACGTGAACTTTCACGCAATGTTCG252760.218865598027127No Hit
CCACATTCTCATCACTGGTGCTGCTGGTCAAATCGGCTACGCTCTTACAT233050.20179865334792665No Hit
CCGAGAACGACGCAATCATCAAAGCAGCTATCGCCGAAGCAGCCAAGTCT216710.1876498011887114No Hit
CACATTCTCATCACTGGTGCTGCTGGTCAAATCGGCTACGCTCTTACATT210500.18227254464594966No Hit
CTTACATCTTCAGTCTCTCTTCCAGCACGTGAACTCTCCCGCAAGGTTCT189380.1639846769836102No Hit
CAAGCATTCAACAAGAGCAAGAAAGTTCAGGCATTCGTCGACGAGTTCGT169610.14686577813491458No Hit
CGAGAACGACGCAATCATCAAAGCAGCTATCGCCGAAGCAGCCAAGTCTG158360.1371243713545491No Hit
CGACAACCTACTACGACGAGAAATCCGGTTTCAGGATGCAGATCCACTTC153850.1332191496141537No Hit
TCCACATTCTCATCACTGGTGCTGCTGGTCAAATCGGCTACGCTCTTACA151340.13104573352360105No Hit
CTCACATCTGTTAGCTATCCAGTCCGCAACATCTGCAGAAGCAAGCTCCC139370.12068087670929217No Hit
GTTAAAGTTGCTATCAACGGTTTCGGCAGAATTGGCCGTCTTGTTTTCCG133580.11566729935299741No Hit
CTTCAGTCAACTTCCCAGCACGTGAACTTTCACGCAATGTTCGCCCAACC131470.1138402443924133No Hit
CAAGCTTACACTTCACGACCTTACAGAGAAAGATCTCGCCGGCAAGCGCA131390.1137709721664196No Hit
CAGGCATTTAACAAGAGCAAGAAAGTTCAGGCATTCGTCGACGAGTTCGT130170.11271457072001552No Hit
AGAAGACGTTCAGACACTCGTTATCGATAACGGCTCTGGTATGTGCAAGG129270.11193525817758629No Hit
TCCACATCCTCATCACCGGTGCTGCTGGCCAAATCGGCTACGCTCTTACA125470.10864482744288506No Hit
CTTTACTGTCGCCCAGATCCGCGAAGTTATGAACAAGCGTCATAACATTC123450.10689570373654388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGAG30100.054.5827941
CCTACTA32900.049.7920467
AACCTAC35400.048.81935
GTGGAGC33750.048.3851622
GGAGCGA33700.048.3234184
ACCTACT35100.047.3428466
TGGAGCG36100.044.716233
TACTGTC31000.041.658224
GAGCGAT39350.041.0230265
AGCGATC40250.040.1057366
CCTGACG21150.039.060016
GGTAGTT23150.037.535851
CTGTTAG39150.036.1476148
GTTTGAC18600.035.485261
CAACCTA48950.035.4024734
GTCTGAG14750.034.7678571
GACGTAC23850.034.245929
CCGATCC22500.033.762213
CTGACGT24800.033.506047
TGACCTG24800.033.3112563
Produced by FastQC (version 0.11.2)