FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCB20_1_CTTGTA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCB20_1_CTTGTA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11548640
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGATTTCATTACCTTCTTTCTTAACGAAGAAAAGAGCTGGAATTGAAG268060.23211391124842407No Hit
GTCAACACGAAGTTCGTTGTAGAATGTGTGGTGCCAGATCTTTTCCATAT127180.1101255212735006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTACC62550.040.5469369
CCCGTCC500.001612516838.018711
CTCGGTT17950.037.8598631
TCATTAC71800.036.31558
CGGGTTA2550.033.5459251
CCCTATG2200.033.46600766-67
CGGGTGT8400.032.2480161
CGGGGCA1805.456968E-1231.682261
CCGCTAT8300.029.7736931
CGGGGTG1652.6393536E-928.8020571
CCCGTGT2151.8189894E-1228.7350751
CGGGCCC856.392288E-427.9549371
CGCGAAA7200.027.721981
CGGGACG700.00837452427.1562251
CCCGGTA1056.888036E-527.1562231
AAAGCTA37450.026.632484
CGGTTTT30350.026.2919583
CGGGGAT6750.026.0498621
CCCGCTA3850.025.921851
CCGGGGT1701.1193151E-725.1594451
Produced by FastQC (version 0.11.2)