FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCA04_3_CAGATC_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCA04_3_CAGATC_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12990625
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGTTCCAATTTACGAAGGCTACTCCCTTCCACACGCCATCATGAGAC275500.21207601635795043No Hit
CAGACACTCGTTATCGATAACGGCTCTGGTATGTGCAAGGCTGGCTTCTC155300.11954775078181382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCGC16850.048.7484055
AGACCCG17750.046.8114664
CGGACTA7600.046.230724
CGTTCGA20150.042.8853454
CCTGACG19750.039.666396
CCCGCTG21950.038.2909777
AACCTAC17500.035.5424585
ACCCGCT23600.035.006056
ACCTACT15350.034.0243766
ACCTGAC24100.032.5073175
CCTACTA15450.031.9641087
GACGTAC25450.031.1584879
CCTCGGG6600.030.57584414-15
GACCTGA27000.029.894944
GCCCTAT8250.027.65275466-67
CGAGCCC8300.027.19964462-63
AGCCCTA8850.026.58355164-65
CTGACCC24750.025.514813
CCCTGAC10300.025.36322838-39
CCCTATG9000.025.34835666-67
Produced by FastQC (version 0.11.2)