FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCA04_3_CAGATC_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCA04_3_CAGATC_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12990625
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGTTCCAATTTACGAAGGCTACTCCCTTCCACACGCCATCATGAGAC184960.14237960067356265No Hit
CTTGTCGATACCATCGTATTCCATACCATCGAAGTATGGCTTGAAGAGCA140710.10831657445273034No Hit
GTCGATACCATCGTATTCCATACCATCGAAGTATGGCTTGAAGAGCATTT131140.100949723358191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGGG6150.035.91419614-15
GGACCGG11400.035.415425
CTCGGGG6250.035.33902716-17
CCCTATG8050.032.45239666-67
CGAGCCC8200.031.85863362-63
CCGGGCT2300.030.9986461
GCCCTAT8900.029.08616466-67
CGGGTCG700.00836780427.1607171
CGGGCCC2650.026.9044861
GACCGGC14400.026.717816
TCGGACG10250.025.25788390-91
CTCGGTT22700.025.1266541
CGGGGAA11000.023.74925418-19
CGGGGCT4900.023.2806151
CCAGGGA19850.022.9714815
CACAGTT109250.022.8846131
TCGGGGA11450.022.81596416-17
GGGCTTA8650.021.4155124
AGCCCTA12050.021.28555764-65
CGGGGGT3850.020.9878271
Produced by FastQC (version 0.11.2)