FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCA04_2_CAGATC_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCA04_2_CAGATC_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13534767
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACACTCGTTATCGATAACGGCTCTGGTATGTGCAAGGCTGGCTTCTC181030.133751840722489No Hit
GAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCTATCA136460.10082183165768571No Hit
CACAGTTCCAATTTACGAAGGCTACTCCCTTCCACACGCCATCATGAGAC136300.10070361757982238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGACG21850.044.540456
CGGACTA9800.044.0824744
GACCCGC21450.040.0593155
CGTTCGA22700.039.9446074
AGACCCG22450.037.428944
AACCTAC23000.036.7404445
CCTACTA19750.036.3032537
ACCTACT20350.035.9259726
ACCTGAC28600.035.1902285
GACGTAC29100.034.2672849
GACCTGA31550.032.0502054
CTGACGT31100.031.6042657
CTGACCC25650.031.0937083
CCCGCTG27500.031.0796097
CGTACTG16450.028.8648857
GGGGACT7950.028.0660513
CTGTTAG35500.027.8231328
CGTGGAG37100.027.639621
TGACGTA37500.027.478918
ACCCGCT31750.027.362746
Produced by FastQC (version 0.11.2)