FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCA04_2_CAGATC_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCA04_2_CAGATC_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13534767
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCCC1401.0913936E-1137.34731
CCTCGGG5500.034.11250714-15
CCGGGCT2100.033.9520951
CTCGGTT26950.033.334781
CGGGTCG600.00393905831.6886181
CGGGGCA2600.031.0792241
CTCGGGG6150.030.12078316-17
CCCTATG6900.029.94482666-67
CCGGGAT10350.028.4738331
CCGGGCA2700.028.1676621
CGGGGGT3900.028.032241
CCGGGTT6800.027.9605461
CGAGCCC7550.027.681262-63
GCCCTAT8400.025.16299666-67
GGACCGG15900.023.599545
CGGGGCT5950.023.1673091
CGGGCTA1852.5288864E-723.1241261
TCGGTTT43000.021.6518462
CCGTCCA23050.021.6372264
CGGGGAT8800.021.6058791
Produced by FastQC (version 0.11.2)