FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCA04_1_CAGATC_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCA04_1_CAGATC_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10207414
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGTTCCAATTTACGAAGGCTACTCCCTTCCACACGCCATCATGAGAC141880.13899700746927676No Hit
CAGACACTCGTTATCGATAACGGCTCTGGTATGTGCAAGGCTGGCTTCTC140260.13740992576572283No Hit
GAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCTATCA108310.10610914772340968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA16650.042.770154
CCTGACG15950.041.968796
GACCCGC14350.040.0314645
AGACCCG14400.039.562394
CGGACTA9050.037.7700584
ACCTACT14050.036.4934926
GACCTGA19950.034.0296024
AACCTAC16200.033.7016265
ACCTGAC20350.033.5943375
CCCGCTG17500.032.8295067
CCTACTA15100.032.0730447
CTGACGT21400.031.9496217
GACGTAC21850.030.6419669
ACCCGCT19450.030.511146
CTGACCC16600.027.7412033
TGACGTA26950.026.078198
CCGATCC19150.025.286753
CTGTTAG29250.023.865258
CGGACCT9100.022.9547673
CCTCGGG3200.022.2561914-15
Produced by FastQC (version 0.11.2)