FastQCFastQC Report
Thu 11 Sep 2014
SDW_NYCA04_1_CAGATC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_NYCA04_1_CAGATC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10207414
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACG857.190465E-944.735031
CTCGGTT18100.028.0984211
CCGGGAT5350.026.6528821
CCTCGGG2850.025.830314-15
CCGGGCC1309.980575E-625.5936011
CGGGGAT6400.025.250831
CCGGGGC950.00122256325.01631
CCGGTAT7050.024.9453331
CGGGGGT3050.024.934281
CGGGCCC1151.2770569E-424.7987691
CTCGGGG3100.023.7472116-17
GGACCGG9500.023.4968935
CCGGGTT5150.023.0732861
CGGGGTT6900.022.7322041
AGGGACG12150.022.6718989
GGCAGGG12650.021.7757766
CGGGGCT3950.021.6596851
CGAGCCC4200.021.48883262-63
CCGGGTA2951.3278623E-1020.945851
CCGCTAC27600.020.8378541
Produced by FastQC (version 0.11.2)