FastQCFastQC Report
Thu 11 Sep 2014
SDW_GOR69_3_CGATGT_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_GOR69_3_CGATGT_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13744063
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACACTCGTTATCGATAACGGCTCTGGTATGTGCAAGGCTGGCTTCTC280600.20416088022879408No Hit
CACAGTTCCAATTTACGAAGGCTACTCCCTTCCACACGCCATCATGAGAC176000.12805529194678458No Hit
GAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCTATCA166900.12143425128362698No Hit
CTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCTATCATCACACAACA152660.1110734140261144No Hit
CTTCAGTCAACTTCCCAGCACGTGAACTTTCACGCAATGTTCGCCCAACC141220.1027498200495734No Hit
TTCAGACACTCGTTATCGATAACGGCTCTGGTATGTGCAAGGCTGGCTTC138260.10059616286683201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCGC19050.050.839025
AGACCCG21850.045.1931154
CGGACTA10000.040.353334
CTGACCC22450.038.6988683
CCCGCTG25150.038.5134747
CGTTCGA22600.036.971294
ACCCGCT27800.036.3735546
CTGTTAG41900.032.7503058
AACCTAC20850.032.5605665
ACCTACT17900.031.030146
CCTACTA18450.030.1099457
CCTGACG14300.027.8865076
CCGATCC20150.026.8592193
CCTCGGG5100.025.1369914-15
CGAGCCC5900.024.97127362-63
TAGTGCG6700.024.8002913
TCTGACC39850.024.7790342
GCGGACT17000.024.5752123
GACGTAC15750.024.4180369
TTCAGTC121900.024.2623962
Produced by FastQC (version 0.11.2)