FastQCFastQC Report
Thu 11 Sep 2014
SDW_GOR69_3_CGATGT_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_GOR69_3_CGATGT_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13744063
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTT32250.043.034541
CGGGACG1102.72308E-630.2460981
CCGGGAT8300.029.777571
CCTCGGG3950.029.46171214-15
TCGGTTT53300.027.3608882
CCCTATG4700.027.28654966-67
CTCGGGG4500.025.3327416-17
GGTTTTA59000.025.2788414
CGGTTTT56450.024.9080923
CAACCGC17250.024.506458
CGAGCCC5600.022.90112762-63
ACAACCG19100.022.6301567
GCCCCGC753.0370671E-522.1661148-49
TAGTATG61950.021.7750789
CCAGGGA13100.021.7550185
CACACCC7150.021.2596763
CCGGGTT6150.020.8666461
CCGCTAC18450.020.3514211
GTTTATT129200.020.3435461
CCGGGCC1656.29893E-520.1640661
Produced by FastQC (version 0.11.2)