FastQCFastQC Report
Thu 11 Sep 2014
SDW_GOR69_2_CGATGT_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_GOR69_2_CGATGT_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10519443
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGTTCCAATTTACGAAGGCTACTCCCTTCCACACGCCATCATGAGAC188590.1792775530035193No Hit
GAACGATCAGCTCGTCTCTCTTAAGACAGAATCCTCTGGCTGCGCTATCA125040.11886560913919111No Hit
CAGACACTCGTTATCGATAACGGCTCTGGTATGTGCAAGGCTGGCTTCTC110910.10543333900853875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCGC11800.045.869695
AGACCCG13650.038.6088684
CGGACTA6850.038.12144
ACCCGCT15750.035.2702266
CCCGCTG16200.033.121647
CCTCGGG6250.031.14854614-15
CGAGCCC6950.030.42702362-63
CTGACCC19000.029.7365153
AACCTAC14500.029.4698165
CCGATCC16400.028.371283
CCCTATG7650.027.64278666-67
ACCTACT13600.027.5798026
TCGGGGA7650.027.31028416-17
CCTACTA13900.026.6457677
CCCTGAC7850.025.71518138-39
CGAGCCG3750.025.3214911
CTCGGGG7700.024.97464216-17
CGTTCGA16000.024.3327184
GCCCTAT8900.023.49340266-67
AGCCCTA8950.023.36270964-65
Produced by FastQC (version 0.11.2)