FastQCFastQC Report
Thu 11 Sep 2014
SDW_GOR69_2_CGATGT_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSDW_GOR69_2_CGATGT_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10519443
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGTTCCAATTTACGAAGGCTACTCCCTTCCACACGCCATCATGAGAC123290.11720202295882015No Hit
CTTGTCGATACCATCGTATTCCATACCATCGAAGTATGGCTTGAAGAGCA108540.10318036800997925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTT27900.040.7191621
CCTCGGG4650.037.79405614-15
CCCTATG5600.036.47249266-67
CGGGGCT3000.036.4427951
CCGGGAT6750.033.8020131
CTCGGGG5200.032.8829516-17
GCCCTAT6800.031.7824666-67
CGGGACG753.0672125E-431.6893881
CCGGGAG1401.6969352E-830.5576251
CGGGGCA2400.029.7088011
CGAGCCC7000.029.51664262-63
TCGGTTT40600.029.0155852
TCGGACG7150.028.90290690-91
CCAGGGA14250.028.6651485
CGGGGTT4700.028.3181761
CGGTTTT41700.028.0216943
CCCGGCT2053.092282E-1127.824831
CGGGTCG700.00836530927.1623341
CGGGGTG1606.2085746E-826.7379231
AGCCCTA7750.026.66044864-65
Produced by FastQC (version 0.11.2)